Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Oxymatrine Protects Chondrocytes against IL-1 β -triggered Apoptosis in Vitro and Inhibits Osteoarthritis in Mice Model

doi: 10.1155/2022/2745946

Figure Lengend Snippet: Impact of OMT on the IL-1 β -triggered apoptosis in chondrocytes. (a) Western blot results of cleaved-caspase-3, Bcl-2, Bax. (b) Results of flow cytometry. ∗ P < 0.05 and ∗∗ P < 0.01 vs IL-1 β -induced group; # P < 0.05 and ## P < 0.01 vs control group.

Article Snippet: The CCK-8 kit was supplied by Beijing Solarbio Technology Co. Ltd. Antibodies specific for β -actin (Abcam, # ab8227), Bax (Bioss, # bs-0127R), type II collagen (Bioss, # bs-11929R), Bcl-2 (Bioss, # bs-0032R), type X collagen (Bioss, # bs-20085R), cleaved-caspase-3 (Abcam, # ab214430), cleaved-caspase-9 (CST, # 9507S), I κ B α (CST, # 9242S), p-I κ B α (CST, # 2859T), p65 (CST, # 8242T), p-p65 (CST, # 3303T), MMP-13 (Proteintech, # 18165-1-AP), and MMP-9 (Proteintech, # 10375-2-AP) were used in this study.

Techniques: Western Blot, Flow Cytometry

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Oxymatrine Protects Chondrocytes against IL-1 β -triggered Apoptosis in Vitro and Inhibits Osteoarthritis in Mice Model

doi: 10.1155/2022/2745946

Figure Lengend Snippet: TUNEL and IHC staining for BCL-2 and Bax show the effect of OMT on progression of ACLT-triggered OA mice. (a) TUNEL results of mice in each group 8 weeks following surgery. Scale bar: 100 μ m. Right: results of statistical analyses of apoptotic chondrocyte rate. (b) IHC staining for BCL-2 and Bax expression of mice in each group 8 weeks following surgery. Scale bar: 100 μ m. Right: results of statistical analyses displayed as the percentage of Bcl-2 or Bax-positive expression area within the total area. ∗ P < 0.05, and ∗∗∗ P < 0.001 vs vehicle group; # P < 0.05 and ### P < 0.001 vs sham group.

Article Snippet: The CCK-8 kit was supplied by Beijing Solarbio Technology Co. Ltd. Antibodies specific for β -actin (Abcam, # ab8227), Bax (Bioss, # bs-0127R), type II collagen (Bioss, # bs-11929R), Bcl-2 (Bioss, # bs-0032R), type X collagen (Bioss, # bs-20085R), cleaved-caspase-3 (Abcam, # ab214430), cleaved-caspase-9 (CST, # 9507S), I κ B α (CST, # 9242S), p-I κ B α (CST, # 2859T), p65 (CST, # 8242T), p-p65 (CST, # 3303T), MMP-13 (Proteintech, # 18165-1-AP), and MMP-9 (Proteintech, # 10375-2-AP) were used in this study.

Techniques: TUNEL Assay, Immunohistochemistry, Expressing

Journal: Antioxidants

Article Title: Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats

doi: 10.3390/antiox9080679

Figure Lengend Snippet: Western plot ( A ) and immunohistochemistry ( E–F ) of myocardial caspase 3, and PARP and apoptosis TUNEL stain in IR myocardium. The statistic data in Caspase 3 ( B ), PARP ( C ) and TUNEL positive nuclei ( D ) are indicated. Data are expressed as mean ± SEM ( n = 3) using the single values. * p < 0.05 vs. CON. a p < 0.05 vs. STZ. b p < 0.05 vs. IR CON.

Article Snippet: The expression of β-actin (#4970, 1:1000; Cell Signaling Technology, MA, USA), 4-HNE (bs-6313R, 1:1000; Bioss, MA, USA), BAG3 (NBP2-27398, 1:2000; Novus, CO, USA), Bcl-2 (bs-0032R, 1:1000; Bioss, MA, USA), caspase 3 (bs-0081R, 1:1000; Bioss, MA, USA), p-eNOS (ser1177, 1:1000; Cell Signaling Technology, MA, USA) and PARP (#9532, 1:1000; Cell Signaling Technology, MA, USA) from the damaged heart infarct areas after ischemia/reperfusion injury were evaluated by Western blotting as described before [ ].

Techniques: Western Blot, Immunohistochemistry, TUNEL Assay, Staining